Dimetilformamida adicionada no sêmen de caprinos sobre a longevidade e funcionalidade da membrana espermática após criopreservação

Wildelfrancys Lima  Souza; Elenice Andrade  Moraes

doi:10.31416/rsdv.v5i1.136

Authors

Wildelfrancys Lima Souza Universidade Federal do Vale do São Francisco, Centro de Pesquisa em Suínos, Espécies Nativas e Silvestre-CPSENS.
Elenice Andrade Moraes Universidade Federal do Vale do São Francisco, Colegiado de Zootecnia.

DOI:

https://doi.org/10.31416/rsdv.v5i1.136

Keywords:

hyposmotic, plasma membrane, spermatozoa

Abstract

Aimed to evaluate the effect of dimethylformamide associated or not with glycerol in diluent of goat semen before cryopreservation on longevity and functionality of the plasma membrane. Ejaculated four goats were collected and subjected to the following treatments: Control; 2% of dimethylformamide (DMF2); 3% of DMF (DMF3); 4% of DMF (DMF4); 5% of DMF (DMF5); and 2% of DMF plus 2% of glycerol (DMF2G). After cooling to 5 °C/2h,
packed and placed under nitrogen vapor for 7 min, then immersed in liquid nitrogen and soon after, thawed at 37 °C/30 seconds. Samples were analyzed for longevity and hyposmotic test. The variables were evaluated with ANOVA and Tukey's test (P <0.05). Sperm motility was higher over the 120 min of TTR in the samples treated with DMF2, when
compared to the other concentrations (P <0.05). It was observed that spermatozoa treated with DMF2, presented higher percentage of cells with conservation in the functionality of the plasma membrane when compared to the other treatments (P <0.05). Thus, the use of 2% dimethylformamide in diluent of semen goat before cryopreservation it provides beneficial effects to spermatozoa after thawing, it is indicated as substitute of glycerol in cryopreservation of spermatozoa goats.

Author Biographies

Wildelfrancys Lima Souza, Universidade Federal do Vale do São Francisco, Centro de Pesquisa em Suínos, Espécies Nativas e Silvestre-CPSENS.

Graduado em Zootecnia na Universidade Federal do Vale do São Francisco. Mestre em Ciência Animal-Univasf. Tem experiência na área de Zootecnia, com ênfase em Reprodução Animal, atuando principalmente nos seguintes temas: criopreservação espermática e nutrição animal.

Elenice Andrade Moraes , Universidade Federal do Vale do São Francisco, Colegiado de Zootecnia.

Graduada em Zootecnia pela Universidade Federal de Viçosa (2002), Mestrado (2004) e Doutorado (2008) em Zootecnia na aréa de Fisiologia e Reprodução Animal pela Universidade Federal de Viçosa (2008). Doutorado Sanduiche na Colorado State University (2006-2007). Pós-Doutorado em Zootecnia na aréa de Reprodução Animal pela Universidade Federal de Viçosa (2008-2009). Estagio Pós Doutoral pela CAPES na Colorado State University na área de Reprodução Animal e Biologia Molecular (2009). Atualmente é Professora Adjunto IV no Colegiado de Zootecnia da Fundação Universidade Federal do Vale do São Francisco (UNIVASF). Professora responsável pelo setor de produção de Suinocultura. Coordenadora Responsável pelo Centro de Suínos, Espécies Nativas e Silvestres (CPSENS)/FINEP-Univasf. Professora Permanente no Programa de Pós-Graduação em Ciência Animal (CPGCA) na Univasf. Ministra as disciplinas na área de Suinocultura, Equideocultura e Cunicultura na Graduação, e Coordenada as disciplinas de Seminário I e Técnicas Reprodutivas em Equinos na Pós-Graduação. Atua em diversas Comissões na Univasf: Comissão Permanente de Controle Disciplinar (CPCD), Comissão Permanente de Progressão Docente (CPPD), Comissão Gestora Permanente de Concurso Docente (CGPCD), Comissão Própria de Avaliação de Curso (CAC-CPA) e do Núcleo Docente Estruturante do Colegiado de Zootecnia (NDE).

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